Nucleoside Modified mRNA Vaccines for Infectious Diseases
[...] FPLC purification and
substitution of modified nucleosides in the mRNA make it non-inflammatory and highly translatable(Kariko et al., Immunity 23:165-175, 2005; Kariko et al., Mol Ther 16:1833-1840, 2008; Kariko et al., Nucleic Acids Research 39:e142, 2011) that are crucial features for therapeutic relevance.
Formulation of the mRNA in lipid nanoparticles (LNPs)protects it from degradation enabling high levels of protein production for extended periods of time (Pardi et al., J Control Release, 2015).